Is it possible to do mTOR K-LISA activity test without immunoprecipitation after cell lysis?

We want to perform a K-LISA (ELISA for kinase activity) for mTOr, but we do not have many cells. So we would like to do it without the immunoprecipitation step, is it possible? Also, how many ug...

26 January 2016 7,365 3 View