Ana María Bravo Valencia

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Questions related from Ana María Bravo Valencia

MTT assay in human lymphocytes: seeding density & RPMI precipitation issue?

In the MTT assay with human lymphocytes (96-well plates, suspension cells), is 70,000 cells/well within the optimal linear range? Also, when using RPMI with phenol red, I observe MTT precipitation...

09 August 2025 8,809 1 View

How many cells and how long to incubate XTT in human PBMC cytotoxicity assays with natural extracts?

I am using the XTT Cell Viability Assay Kit (Biotium, Cat. No. 30007) to evaluate the cytotoxicity of a natural extract in human peripheral blood lymphocytes. For those who have experience with...

09 August 2025 480 0 View