Have a PCR product obtained with degenerate primers, need to clone. Interesed in one band specifically, how do I choose the ratio product:vector?

I am using degenerate primers to amplify a family of genes. My results in an agarose gel are 6 bands (862pb, 632pb, 408pb, 335pb and 226pb). The bands of interest are 408pb and 335pb. What...

28 November 2017 7,448 3 View