An Nguyen

2 Questions 11 Answers 0 Followers

Questions related from An Nguyen

Can anyone help me with expression and purification of GST-tagged protein?

I want to express and purify a protein of bacteria (approximately 121 kDa) using pGEX 4T-1, E. coli BL 21 and Glutathione Sepharose 4B. I have tried out with 0.1, 0.5 mM IPTG, at 30 deg C for 4...

01 January 2019 9,164 4 View

How to analyze quantitative realtime PCR result in order to compare expression of 1 gene between bacteria A and B?

Hello everyone! I want to compare expression of 1 gene (example: Z) between bacteria A and B by quantitative realtime PCR using SYBR green. - First, I made a standard curve for each bacteria by...

12 December 2018 7,695 13 View