Amy L Roberts

2 Questions 6 Answers 0 Followers

Questions related from Amy L Roberts

Are there any drawbacks of using Peak Area instead of Peak Height to calculate ratio of alleles in fragment analysis?

I am setting up a fragment analysis assay in which I need to calculate the ratio of the two peaks. The protocol I have inherited suggests using Peak Height, and I see this used a lot generally for...

08 May 2019 9,491 1 View

Is a polyacrylamide gel better than a high resolution agarose gel at visualising faint bands of digested products?

I am using the transgenomics surveyor assay to detect the presence of mismatched DNA after hybridisation. Firstly I amplify a PCR product of 300bp. Then I denature and hybridise this DNA, which,...

17 April 2012 6,437 15 View