Amy Replogle

2 Questions 5 Answers 0 Followers

Questions related from Amy Replogle

Does anyone have any recommendations for housekeeping genes to use in qPCR for rat skeletal muscle?

I have a student who is comparing exercising rats (that had voluntary access to running wheels for 8 weeks), sedentary rats (that were in wheel-free cages for 8 weeks), and de-trained rats (that...

03 March 2015 8,575 4 View

How do I properly normalize T-DNA insertion lines with reference genes?

If I'm doing a qPCR experiment where I need to determine the relative decrease in gene expression from RNAi lines that were generated by T-DNA insertion do I need to run my reference primers on...

18 February 2015 6,808 3 View