Has anyone seen negative mP at high concentrations of inhibitor in fluorescence polarization competition assay?

Hi, I am doing a competition assay: {protein] is 250 nM and [fluorophore} is 30 nM (the Kd of the interaction is 250 nM). The highest concentration of inhibitor is 10 uM and I'm doing a 1 in 2...

28 February 2016 6,695 13 View