I am looking for antigen retrieval and reverse crosslinking protocols for immunostaining in C. elegans adults. Can somebody advise me on the same?

I am staining for neuronal microtubules in C. elegans strains expressing GFP-tagged beta-tubulin, using anti-GFP antibody (12A6, supernatant- DSHB). I observe no staining in any of my samples...

28 August 2019 2,537 2 View