Amruta Deshpande

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Questions related from Amruta Deshpande

Why does the a lane of betactin bands appear on the top of membrane, where there is well bottom surface where sample is loaded?

I am carrying out western blot analysis to determine pro-inflammatory markers in LPS induced murine RAW 264.7 macrophage cell lysates. I am using beta actin as house keeping protein and optimizing...

10 November 2021 9,193 2 View

While using ibind system for western blotting and multiplexing when is the primary Ab conjugated with fluorochrome added to the system?

I am carrying out Western blot to determine effects of extracts on expression of LPS induced iNOS in murine macrophage cells, I am using the iBind flex system from Thermofisher for multiplexing...

04 August 2021 7,085 0 View