Amruta Bapat

4 Questions 2 Answers 0 Followers

Questions related from Amruta Bapat

I am planning to express my protein in Pichia and would like recommendations on the best expression kits or individual components for expression?

I have no prior experience with expression in Pichia. I would like recommendations on individual Pichia cells and vectors, or expression kits. I also want to make decisions about secreted /...

20 July 2020 1,369 1 View

Can anyone suggest an ideal buffer for commercial PME (from orange peel) as a positive control for the assays mentioned below?

I have 11 mg of commercial PME from orange peel. I would like to use this as a positive control for two different assays which I am currently standardizing. The assays and the buffers used for...

20 August 2019 4,858 0 View

I would like to take bacterial OD600 measurements on a microplate reader? How can I do that?

I understand the OD measurement in a plate reader varies with the volume of the sample measured. Is there a formula to correct for path length without finding the correction factor by myself?

04 June 2019 7,437 1 View

Is pET21 vector compatible with Rosetta host cells?

pET21 contains ampicillin gene as the selectable marker and Rosetta cells require Cloramphenicol because of the pRARE plasmid? What antibiotic should I use then?

17 May 2019 4,300 3 View