Amol J Patil

5 Questions 9 Answers 0 Followers

Questions related from Amol J Patil

Is there any limitation on amount of broth to be taken while growing overnight culture of E.Coli from a single colony?

I need a maximum possible volume of initial culture. I wish to use ASTM E 2149 method. It consists of adjusting the OD at 0.28 and then use 50 ml. volume for each textile sample. Now, I usually I...

10 March 2015 7,454 4 View

Will I get erroneous results if microliter volumes are used in place of milliliter volumes?

For instance, if I do serial dilution of microbiology culture with 10^-6 with (100/900 microliter) in place of 1mL/9mL volumes, is it acceptable or is it a bad practise?

10 December 2014 3,060 14 View

What is the best diluent for E.coli dilution for cell count? Is it peptone water/saline/deionized water?

In which solution, there are minimum chances of bacterial death and control of the growth at the same time?

12 November 2014 3,355 10 View

Could someone explain the method of obtaining the required E-coli count for antibacterial efficiency test AATCC 100 for fabrics?

The method mentions that the 24h broth culture should be diluted to get 1-2 x 10^5 E-coli cells. My question is how to figure out the relation between dilutions and number of cells? I am sure we...

19 October 2014 8,984 3 View

Are antimicrobial-treated hospital textiles commercially available?

01 January 2014 3,137 2 View