Amol J Patil

3 Questions 6 Answers 0 Followers

Questions related from Amol J Patil

Is there any limitation on amount of broth to be taken while growing overnight culture of E.Coli from a single colony?

I need a maximum possible volume of initial culture. I wish to use ASTM E 2149 method. It consists of adjusting the OD at 0.28 and then use 50 ml. volume for each textile sample. Now, I usually I...

10 March 2015 7,432 4 View

Will I get erroneous results if microliter volumes are used in place of milliliter volumes?

For instance, if I do serial dilution of microbiology culture with 10^-6 with (100/900 microliter) in place of 1mL/9mL volumes, is it acceptable or is it a bad practise?

10 December 2014 3,038 14 View

Could someone explain the method of obtaining the required E-coli count for antibacterial efficiency test AATCC 100 for fabrics?

The method mentions that the 24h broth culture should be diluted to get 1-2 x 10^5 E-coli cells. My question is how to figure out the relation between dilutions and number of cells? I am sure we...

19 October 2014 8,961 3 View