I am using E. coli BL21 (DE3) to express proteins but I am getting problem with lysis. Can someone help me with it ?

I am using E. coli BL21 (DE3) to express proteins but I am getting problem with lysis, sometimes it work and sometimes it doesn't. I am using lysis buffer (50mM NaHPO4, 30mM NaCl, 0.5 Triton-X, pH...

02 February 2016 5,236 5 View