Amiti Amiti

4 Questions 5 Answers 0 Followers

Questions related from Amiti Amiti

can GC% in primer affect the pcr product?

I have a primer where the G% of forward primer is 50% and that of reverse is 20% , and annealing temperature is 48 degree C ,what could be the reason of not getting pcr product? can GC% really...

10 August 2018 2,833 3 View

Can anyone give me correct protocol for TBARS assay to measure lipid peroxidation in RAW 264.7?

I am doing TBARS assay to measure lipid peroxidation in LPS induced RAW 264.7 cells.I am not getting any result as there is no appearance of pink color in sample after doing assay.I am following...

13 July 2016 3,873 5 View

Pcr of repetitive region

I'm trying to do a pcr of repetitive region, however with non fluorescent tagged primer I am getting the correct pcr product but when I tagged my primer with hex dye I am getting product above...

01 January 1970 4,160 3 View

Protein extract from mice's pancreas

urgently i want to extract protein for western blot from pancreatic tissue of mouse. for that i m using RIPA buffer and PMSF? whether adding PMSF is sufficient? currently, PMSF is only available...

01 January 1970 5,065 3 View