Amit K Dash | Baylor College of Medicine

Amit K Dash

2 Questions 7 Answers 0 Followers

Questions related from Amit K Dash

What should be the annealing temperature in PCR working with a low template DNA conc.(50ng/microlt.). Should it be decreased compared to the usual temperature?

DNA conc. (50ng/microlt.)

01 January 2013 400 3 View

Genomic DNA of conc 50 and 70ng/microlit (dissolved in TE pH 8.0) did not amplify in PCR, but DNA of 100ng/microlit dissolved in H2O got amplified.

Is EDTA interfering? If I dissolve this DNA in water for PCR, will it be amplified, since DNA conc. will dilute?

01 January 2013 6,011 12 View