Amit Kumar Verma

2 Questions 5 Answers 0 Followers

Questions related from Amit Kumar Verma

Can polyclonal antibodies crossreact against tagged proteins?

I have cloned my protein into pET32 a + vector which contains thioredoxin tag, His tag and S-tag. I want to use this protein for polyclonal antibody generation in rabbits. Can polyclonal...

13 August 2013 8,522 16 View

How to elute sticky His tagged protein from bead?

Hi. I've my protein cloned in pET28 vector which expresses well. While extracting it from bacterial culture, I get very low amount of protein in elution fractions. When I analyse different steps...

01 January 1970 6,941 4 View