Amit Kumar Pradhan

6 Questions 8 Answers 0 Followers

Questions related from Amit Kumar Pradhan

How long can we store dsDNA after extraction from the plant tissue which will be used for marker based genotype screening?

A large of samples needs to be screened using pcr based markers. therefore, the DNA if extracted from the plant tissues how long can it be stored? Can it be stored in -80 or -20, please suggest.

21 August 2020 7,035 3 View

How to increase the intensity of bands in sqRT Pcr analysis for gene expression?

I have tried increasing the no. of cycles as well as checked the annealing temp but still i get a faint bands wherein i m suppose to get bright ones. even it appears same in case of housekeeping...

24 October 2018 1,432 2 View

What might be the reason that during quality using 0.8% gel electrophoresis i got a single thick band but not two separate bands as its seen normaly?

I extracted RNA from the fresh tissues using RNA kit form GCC Biotech, normally during the Quality check using electrophoresis it showed Double band but today in my case i could get only one...

05 September 2018 9,176 3 View

What is the required RNA concentration for cDNA synthesis to undergo semi quantitative RT-PCR?

Minimum concentration required.

06 August 2018 4,597 1 View

How long can we store the plant tissue sample after acid digestion?

I wish to digest the root sample of a plant i.e. in dried form using HNO3. how long can i store it before ICP analysis.

25 May 2018 8,718 4 View

Hello I am trying for untargeted metabolite profiling in rice roots under abiotic stress. Please can you suggest me what should i do initially?

Before I go for LC-MS i wish to do initial check and have a view of probable metabolites which would help me in getting better result.

25 April 2018 9,219 4 View