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Questions related from Amit Kumar
I have run peptide-DNA (single stranded) complex simulation. I understand that the helical structure of ssDNA can not be intact during simulation. But, I wonder how to determine the most feasible...
27 March 2021 1,038 2 View
My protein of interest has ATP and Mg2+ in its active site. In crystal structure, ADP and BeF3 are present in place of ATP. Is it acceptable to build ATP in PyMol out of ADP and BeF3?
30 July 2019 473 2 View
I am examining the inhibition potential of a compound on purified protein. I performed this at seven different concentrations of compound with four conc'ns of substrate1 while maintaining the...
11 March 2016 1,711 6 View
I have cloned a gene and tranformed it to E.coli BL21. Expressed protein are getting into inclusion body. Please help me to troubleshoot this problem.
05 October 2015 1,721 8 View
I would like to know the salt (Nacl) effect at different concentrations on dH and dS of protein ligand interactions in Isothermal Titration Calorimetry. And how pH of buffer (both acidic and...
07 April 2015 9,097 5 View
In my ITC run (Nano ITC, TA instruments), the heat of reaction is less than heat of dilution. For e.g. the heat of reaction for 1st and 2nd titrations was 4.17uJ and 6.23uJ respectively whereas...
28 March 2015 2,338 8 View
I was trying to dissolve ciprofloxacin (antibiotic) in PBS at 100mM concentration. But it was not completely dissolved and at the same concentration, it was completely dissolved in dH2O. Could...
25 March 2015 1,458 21 View
There are missing residues in the crystal structure of my protein. One part of this missing residue region (30 residues length) has no template. When I modelled this protein in modeller by taking...
27 May 2014 7,211 2 View
