Amit Kumar

6 Questions 12 Answers 0 Followers

Questions related from Amit Kumar

How do we predict the best folding of ssDNA from MD simulation analysis?

I have run peptide-DNA (single stranded) complex simulation. I understand that the helical structure of ssDNA can not be intact during simulation. But, I wonder how to determine the most feasible...

27 March 2021 986 2 View

How to calculate Ki for mixed inhibition?

I am examining the inhibition potential of a compound on purified protein. I performed this at seven different concentrations of compound with four conc'ns of substrate1 while maintaining the...

11 March 2016 1,672 6 View

How do I get rid of inclusion body in protein purification?

I have cloned a gene and tranformed it to E.coli BL21. Expressed protein are getting into inclusion body. Please help me to troubleshoot this problem.

05 October 2015 1,678 8 View

How can I neglect heat of dilution from heat of reaction in Isothermal Titration Calorimetry?

In my ITC run (Nano ITC, TA instruments), the heat of reaction is less than heat of dilution. For e.g. the heat of reaction for 1st and 2nd titrations was 4.17uJ and 6.23uJ respectively whereas...

28 March 2015 2,288 8 View

At what concentration can ciprofloxacin can dissolve in PBS completely?

I was trying to dissolve ciprofloxacin (antibiotic) in PBS at 100mM concentration. But it was not completely dissolved and at the same concentration, it was completely dissolved in dH2O. Could...

25 March 2015 1,415 21 View

Why am I seeing secondary structural changes in molecular dynamic simulation?

There are missing residues in the crystal structure of my protein. One part of this missing residue region (30 residues length) has no template. When I modelled this protein in modeller by taking...

27 May 2014 7,178 2 View