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Questions related from Amit Gupta
Dear Experts, As I am doing proteomic profiling for particular fraction of cell. By following the protocol I prepared my sample for LC-MS/MS analysis but the concentration of protein in that...
19 May 2016 7,170 4 View
Dear All, I am planning to do NanoLC-MS analysis, as I am doing this for the first time and I have no idea about sample preparation. I have gone through some of the article and I found they are...
08 June 2015 4,185 4 View
I am following one protocol by that I am trying to take out nucleus proteins but one of my buffer having SDS and in this case I can not use the same sample for 2DE analysis. I tried another...
28 February 2015 4,043 2 View
As I am going on with my proteomic approach. Now, I am stuck at one step I want to look inside the nucleus protein expression profile of different tissues. I am finding some of the kits available...
12 February 2015 8,114 2 View
I am going to do proteomics analysis from cancer tissues. I am doing this for the first time. I am going to deal with solid tumour samples some time I will get fresh tissue. So, to do my job first...
01 January 2015 3,200 4 View
I want to precipitate the chromatin by following antibodies to know what else protein is binding to the site of interest. I have one protocol for Chromatin IP..but by following that I am getting...
14 November 2014 9,603 7 View
I want to check for the different expression levels of p53 protein and I am doing this for the first time. I know about the UV mediated stress for p53 stabilization. So I just want to know if...
17 March 2014 9,409 3 View
