Troubleshooting pcr amplification with touchdown program: can anyone help please ?

Hey guys, I am amplifying an exon in a gene responsible for a hemorrhagic disease I am doing a PCR with touchdown programm : 95° C 10 min -94°C 30sec,69°C 30sec, 72°C 30sec : 7cycles and then...

11 July 2020 1,383 11 View