Amir Mofidi

2 Questions 4 Answers 0 Followers

Questions related from Amir Mofidi

What is the best LPS concentration to stimulate macrophages/monocytes?

I'm going to stimulate the Human machrophages and monoctes with E.Coli LPS, I know 1µg/ml to 10µg/ml LPS could be effective, but I'm looking for the optimal concentration.

02 July 2013 5,717 16 View

How can I design an In Vitro inflammation condition?

To simulate inflammation under In Vitro condition, I'm using DMEM (10%FCS+1%Pen/Srp) combined with 10ng/ml IL-6, TNF alpha and IL-1 beta. Due to the complexity of inflammation and role of...

14 February 2013 8,011 6 View