Amir Jalilvand-Nejad

2 Questions 2 Answers 0 Followers

Questions related from Amir Jalilvand-Nejad

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

14 May 2020 1,777 6 View

How can I digest my recombinant plasmid with xho1 and hind3 enzyms?

Hi, I have tried to digest my recombinant plasmid( PeGFP plasmid 4700 bp and fragment 750 bp) with the following protocol: 1-template= 3 (micro) xho1 =1.5 Hind3=2 buffer=3 H2O=20.5 1 hour in...

14 May 2020 5,332 3 View