Amir Jalilvand-Nejad

4 Questions 3 Answers 0 Followers

Questions related from Amir Jalilvand-Nejad

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

14 May 2020 1,880 6 View

How can I digest my recombinant plasmid with xho1 and hind3 enzyms?

Hi, I have tried to digest my recombinant plasmid( PeGFP plasmid 4700 bp and fragment 750 bp) with the following protocol: 1-template= 3 (micro) xho1 =1.5 Hind3=2 buffer=3 H2O=20.5 1 hour in...

14 May 2020 5,438 3 View

Are common robust counterparts adequate for dependent uncertain parameters?

In most of robust optimization models, uncertain parameters are assumed to be independent. For example Bertsimas and Sym or Ben-Tal and Nemirovski discussed that it is too conservative to assume...

15 April 2014 3,682 1 View

Can anyone introduce a method to model a robust optimization problem based on correlated data in a polyhedral uncertainty set?

Bertsimass and Sim propose a correlated model of data uncertainty in their well-known article (The price of robustness, 2002), but they assume that there are a few sources of uncertainty that...

04 March 2014 8,376 2 View