Amine Bouchareb

4 Questions 23 Answers 0 Followers

Questions related from Amine Bouchareb

Cloning in Dr274 - how to ligate my insert in the BsaI digested DR274?

I'm performing a knock out of a gene in Zebrafish using CRISPR. But i have a problem with ligation. I cannot ligate my insert in the BsaI digested DR274! Protocole used: -Digest backbone DR274:...

03 March 2014 5,877 12 View

What may be wrong with my RT-PCR efficiency?

I'm performing rt-PCR on microRNA, in some reactions I have an efficiency >100 (200%) and in others it's < 100 (65%), I'm working with fast sybrgreen (applied biosystem), cDNA (1/100), primers...

02 February 2014 7,594 5 View

What is the best miRNA knockout technology?

If possible, please give me some information about efficiency and position for the cleavage site (TALEN or CRISPR). Thank you for your answers.

01 January 2014 9,532 3 View

2 step PCR amplicon sequencing - Library without kit

Hi, I'm setting up an experiment where I need to genotype different mouse samples using amplicon sequencing and I'm planning to not use any kit for my library prep. Adapting the 16S Metagenomic...

01 January 1970 4,895 4 View