Amel Kevin Alame-Emane

3 Questions 2 Answers 0 Followers

Questions related from Amel Kevin Alame-Emane

Do CTAB protocol is the appropriate way to extract DNA to perform Mycobacterium tuberculosis whole genome sequencing?

We plan perform Mycobacterium tuberculosis whole genome sequencing of about 1,000 samples, now we're assessing different DNA extraction ways. The first results we got (picture joined) have...

02 February 2018 9,896 3 View

What is the best software to perform Whole Genome Sequencing?

Hi ! In my lab, we plan to do Whole Genome Sequencing (WGS), can someone tell me the best dedicated software for this work? I thought I understood that these software require having super...

05 May 2017 8,340 3 View

In the case of presence of unspecifics bands in a PCR product, can a dilution improve the quality of sequencing ?

I maked some PCR of rpoB gene (Mycobacterium tuberculosis). I obtained my interest fragment of 411 bp together with some unspecifics bands that disturb sequencing step: the qualities of...

03 March 2015 6,527 5 View