Ameerul Moominin Aalam Mohammed

2 Questions 5 Answers 0 Followers

Questions related from Ameerul Moominin Aalam Mohammed

Is alright to reuse the resin in the column for protein purification?

Hello all, I am liitle confused on reusing my resing after purification of protein using gravity flow through column. I am using cobalt resins and I am doin an His-tag purification. So I incubate...

09 September 2017 9,872 5 View

I am having trouble with my cloning, on transforming my ligation to bacteria, incubating overnight at 37 degree i see no colonies suggestion please?

I am trying to ligate 500 BP of my double stranded insert to my vector. I digest my insert with EcoRI and XhoI and I digest my vector with EcorI and SalI. I incubate the ligation reaction at 4...

02 February 2017 6,863 4 View