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I will appreciate if someone advice me with a good protocol for isolation and expansion of MSCs.
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We have stimulated our PBMCs from normal controls with PMA (5-50 ng/ml) and Ionomycin/ 1ug/ml. Our target is to assess the functions of NK cells (both cytokine release and cytotoxicity) in these...
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The yield is always lower and many cells lost specially HPSCs after recovery and many clumbs ? we use 90% FBS and 10% DMSO and put it in Isopropanol freezing box over night in -80Cand transfer it...
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