Mutagenesis PCR Variable Yields Despite Exact Same Conditions???

Hello fellow scientists, I could really use some advice! I'm using a new(ish) Bio-Rad T100 thermocycler to run a mutagenesis PCR using NEB's Q5 Site Directed Mutagenesis kit (E0554S). My PCR...

16 November 2020 2,934 11 View