Is there a problem with buffer as I get no signal with 2D-PAGE or Western blot ?

I am trying to measure phosphorylation of lamin A subunits by 2D-PAGE. I resuspend Vero cells in Rehydration buffer (which contains 8M urea) and sonicate them. I run this cell lysate sample on an...

27 October 2015 3,563 2 View