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Questions related from Amar Thaker
I have this system where when I add HEPES (10 mM pH 8.0) to a solution of lysozyme (6 µM) and gold ion (500 µM) I am able to get gold nanoparticle formation (based on absorption spectra and color...
10 October 2018 8,677 1 View
I have following gel as my Colony PCR result. I see that there is a band between 100 -200 bp. But there is a large amount of DNA in some wells like one in 11 which doesn't migrate. I have seen...
06 June 2018 6,365 4 View
I have this gold nanoparticle - protein complex in solution. I am trying to isolate protein from the gold nanoparticle to retrieve the protein back in solution and centrifuge out the nanoparticle...
04 April 2018 8,383 7 View
These are Maltose Binding Protein crystals and I have to open the seal to make a buffer transfer. The moment I open the wells, within 2 mins the solution turns opaque as shown. Can someone help me...
04 April 2018 2,661 2 View
Hello, I am lysing E.Coli expression strain containing Maltose Binding Protein. I am having trouble in determining the amount of lysis buffer (20mM Tris-HCl pH 7.4 and 0.5 M NaCl) that I should...
03 March 2018 4,769 3 View
I have this soluble and stable protein which I believe to be in complex with gold nanoparticles of small size. My solution contains gold nanoparticles and these proteins bound on its surface. I...
03 March 2018 5,192 3 View
I have a protein-gold nanoparticle complex. As I want to run SDS page on the protein sample, I want to eliminate gold nanoparticles from the sample as it is clogging the gel. Centrifuging at high...
03 March 2018 3,318 6 View
Background: I am currently over expressing Maltose Binding Protein from BL21(DE3) strain of E.coli. For purification I pass it through maltose resin affinity column. Before that I add cell lysis...
01 January 1970 898 7 View