Amar Thaker

8 Questions 10 Answers 0 Followers

Questions related from Amar Thaker

Why does addition of Tris buffer at pH 8.0 inhibits the ability of HEPES buffer at pH 8.0 to reduce gold ions?

I have this system where when I add HEPES (10 mM pH 8.0) to a solution of lysozyme (6 µM) and gold ion (500 µM) I am able to get gold nanoparticle formation (based on absorption spectra and color...

10 October 2018 8,681 1 View

Why does my DNA does not migrate from the well in colony PCR?

I have following gel as my Colony PCR result. I see that there is a band between 100 -200 bp. But there is a large amount of DNA in some wells like one in 11 which doesn't migrate. I have seen...

06 June 2018 6,367 4 View

Which would be the best molecule to bing gold nanoparticle to displace the protein coat?

I have this gold nanoparticle - protein complex in solution. I am trying to isolate protein from the gold nanoparticle to retrieve the protein back in solution and centrifuge out the nanoparticle...

04 April 2018 8,385 7 View

What exactly is happening to my protein crystals when I peel the tape sealing the well ?

These are Maltose Binding Protein crystals and I have to open the seal to make a buffer transfer. The moment I open the wells, within 2 mins the solution turns opaque as shown. Can someone help me...

04 April 2018 2,665 2 View

How much lysis buffer to add and how long sonication required?

Hello, I am lysing E.Coli expression strain containing Maltose Binding Protein. I am having trouble in determining the amount of lysis buffer (20mM Tris-HCl pH 7.4 and 0.5 M NaCl) that I should...

03 March 2018 4,773 3 View

Where did the protein go in this protein-gold nanoparticle complex, understanding through SDS PAGE?

I have this soluble and stable protein which I believe to be in complex with gold nanoparticles of small size. My solution contains gold nanoparticles and these proteins bound on its surface. I...

03 March 2018 5,193 3 View

How to dissolve gold nanoparticles into gold ions in presence of protein?

I have a protein-gold nanoparticle complex. As I want to run SDS page on the protein sample, I want to eliminate gold nanoparticles from the sample as it is clogging the gel. Centrifuging at high...

03 March 2018 3,320 6 View

Can I keep protein extracted from E. coli bacteria (strain BL 21 DE3) in lysis buffer over night after centrifuging and filtering it?

Background: I am currently over expressing Maltose Binding Protein from BL21(DE3) strain of E.coli. For purification I pass it through maltose resin affinity column. Before that I add cell lysis...

01 January 1970 902 7 View