Amar Hundare

3 Questions 5 Answers 0 Followers

Questions related from Amar Hundare

Diagnostic restriction digestion not working on transformed colonies?

I have been trying to transform agrobacterium tumefaciens (LBA4404, EHA105 and GV3101) using my plasmid of interest that harbors a kan resistance gene. I got the colonies on plate with antibiotics...

06 February 2020 4,074 3 View

Colonies of Agrobacterium not growing after transformation using desired vector?

I started liquid culture of two agrobacterium strains(LBA4404 and EHA105) by picking single colonies from selective media of rifampicin. I pelleted the cells and resuspended in fresh media and...

30 January 2020 6,308 3 View

PCR genotyping reproducibility?

I am trying to genotype 14 samples of plant genomic DNA for a particular allele using specific primers. The Tm of primers is 63°C. When I first carried out the PCR reaction for first 7 samples...

14 June 2019 2,500 7 View