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I have been trying to transform agrobacterium tumefaciens (LBA4404, EHA105 and GV3101) using my plasmid of interest that harbors a kan resistance gene. I got the colonies on plate with antibiotics...
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I started liquid culture of two agrobacterium strains(LBA4404 and EHA105) by picking single colonies from selective media of rifampicin. I pelleted the cells and resuspended in fresh media and...
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