Amandeep Singh

4 Questions 7 Answers 0 Followers

Questions related from Amandeep Singh

Can anybody help with a problem in swelling ssDNA cellulose resin?

HelloI am facing problem in swelling the ssDNA cellulose resin in water or Tris-EDTA buffer (10mM Tris pH8.0 and 1mM EDTA). Even after 72 hours it is still slimy/mucoid powder and is blocking the...

01 January 2015 8,337 4 View

Can anyone suggest software for a phylogenetic analysis of a large number of very long sequences?

I am trying to concatenate 8 different genes from 47 different organisms and prepare a phylogenetic tree. Can someone please suggest a good software to handle this data?

02 February 2014 8,867 17 View

How to avoid smear in colony PCR rxn?

I am trying to screen a large no. of clones using colony PCR. I am using the standard and Tm optimized (for my gene) PCR protocol , PHUSION enzyme, other than longer initial denaturation. In all...

09 September 2013 5,044 9 View

Holliday junction prepartion and visualization?

I want to prepare holliday junction. I have synthesized the DNA fragments. I have the mixture to 95 degress and then slowly cool it down. I tried running it on a 15% PAGE gel and stain it with...

04 April 2013 7,136 1 View