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Questions related from Amanda J Stock
We found that specific neutrophil proteins could cleave amyloid beta peptide. My first thought was that the smaller products were probably less toxic than the full amyloid beta 1-42 peptide....
01 January 2017 7,716 2 View
I received 0.5mg of amyloid beta from a company and dissolved it into 0.5ml of 1mM NaOH to make a 1mg/ml solution of amyloid beta 1-42. I then aliquoted the peptide and performed a BCA on the...
06 June 2015 7,626 5 View
Bachem suggests reconstituting the peptide in ammonia or 1mM NaOH to revert aggregation. This sounds like a good idea so the entire batch does not immediately clump together. I was planning to...
09 September 2014 8,687 6 View
I am doing a co-IP to determine if a protein (CAP37) is binding to a receptor. I am using an antibody against the receptor and agarose beads to pull down the receptor, and then doing a WB for...
08 August 2014 3,156 6 View
