Amanda Harmon

4 Questions 2 Answers 0 Followers

Questions related from Amanda Harmon

What could be causing variability of relative expression for subjects between qPCR plate replicates?

So we have been looking at the relative expression of mRNA for an endogenous opioid in 3 different brain regions of our mice using qPCR. For each brain region we want to run two replicate plates...

08 August 2019 957 1 View

What is the recipe for a Gene Expression Assay 20X when you are using custom primers?

So my lab recently switch from TaqMan Gene Expression Assay Mix (20X) to using custom probes and primers from IDT. That being said the primers and probes all came separately instead of as a mix....

08 July 2019 2,572 1 View

What is the recipe for a Gene Expression Assay 20X when you are using custom primers?

So my lab recently switch from TaqMan Gene Expression Assay Mix (20X) to using custom probes and primers from IDT. That being said the primers and probes all came separately instead of as a mix....

07 July 2019 827 1 View

How is DNA microinjected into the cytoplasm of a C elegans gonad region eventually expressed? Is it taken up by the germ line nucleus?

After You inject your DNA sequence into the distal gonad region using the microinjection, what happens to the DNA? Is it taken up by the germ line nuclei during the developmeant of the oocyte? If...

27 March 2018 7,508 3 View