Amanda Fitz

4 Questions 5 Answers 0 Followers

Questions related from Amanda Fitz

Samples are not running down the gel in western blot, could it be loading dye?

hi, this is a 4-12% gradient gel, store bought. I added 20ug of sample to each lane, it is about 2ul volume. The sample seems to get "stuck" at the top of the gel and even though its binding to...

01 January 2016 3,693 6 View

PVDF membrane has streaks when imaging, any ideas?

Hi, I am just wondering if anyone has run into this problem before. I am using the licor imager to try to probe for 2 proteins, Girk and D2. I blocked with the licor blocking buffer 1:1 with PBS....

11 November 2015 8,107 0 View

Western blotting question: Are there any major differences in blocking steps between using the odyssey and a peroxidase conjugated secondary ab?

I recently switched between the odyssey imager (at another location) now to using a standard peroxidase reaction to image my westerns. I notice with the peroxidase reaction I get a serious amount...

10 October 2015 6,748 7 View

Does anyone have a good protocol for Gad65/67 immuno?

I am working with perfused brains that have been sliced on a cryostat at 40um. We are looking for Gad65/67 in cell bodies but Gad antibodies stain almost everything. There is no clear cell bodies....

10 October 2015 4,758 8 View