Which is the best way to stimulate PBMCs for IL-4 detection?

I'm looking for a positive control for IL-4 secretion in a 5 - 7 day PBMC proliferation.  Currently I'm stimulating the cells with CD3 (0.2 ug) in the presence of IL-2 (10 ng) for the culture with...

09 October 2015 169 3 View