Amanda Hill

2 Questions 1 Answers 0 Followers

Questions related from Amanda Hill

DNA concentration for sequencing?

My DNA is about 500 BP and that is about 100 ng/5ul for the DNA mass according to the SMOBIO DNA Ladder. I need a concentration of 2ng/ul for sequencing, and it also says I need 5 ng and 2.5 ul...

31 October 2018 9,871 4 View

My gel is showing two smeared lines very far apart, what could be the cause for this?

I'm looking at primers at different annealing temperatures and my gel is showing two distinct streaking bands at the top of the ladder and at the bottom of the ladder. I'm using DNA from ferns...

12 April 2018 5,869 4 View