Why are my Fura-2 AM 340 and 380 baseline RFU levels so low, but BOTH wavelength RFUs skyrocket when using Ca2+ ionophore?

I am currently evaluating calcium mobilization in suspension human mast cells. My current protocol is to incubate the cells with Fura-2 AM dye (3 uM) in Tyrode buffer (HEPES+BSA, pH 7.4) for 45...

14 March 2018 6,338 6 View