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Questions related from Amanda Chan
I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...
17 March 2024 269 1 View
I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...
22 February 2024 4,762 0 View
If primers amplify a specific region of DNA, and the same DNA is present throughout the body, can a single primer be used across different cell lines and different organs from the same species?
24 January 2024 6,419 2 View
I have been trying to design primers and one of the primer criterion given is that any possible hairpins have a negative delta G value. I am confused about this as I would assume that a more...
12 November 2023 6,181 1 View
