Aman Az

3 Questions 5 Answers 0 Followers

Questions related from Aman Az

What would be the good Cell Membran staining for paraffin embeded liver tumor and melanoma cells?

I am running Immune Fluorescence to locate calreticulin in the cell and I am trying to do double staining where I can stain the cell membrane so calreticulin can be easily located. Any suggestion...

08 August 2017 6,239 0 View

What is the best antibody dilution buffer recipe for fluorescence IHC

I am running a fluorescence IHC to detect calreticulin in paraffin embedded tissue. Primary antibody is polyclonal calreticulin and secondary antibody is Alexa Flour. Using 0.05% Tween 20, 3%...

01 January 1970 6,915 4 View

Does anyone have any idea what is the corrective action for shredded formalin-fixed paraffin embedded section?

I am getting extremely compressed, wrinkled and shredded section. I usually place the block in ice water before sectioning but that did not solve the problem. Microtome used is Leica RM 2125RTS,...

01 January 1970 5,339 15 View