Aman Chahal

2 Questions 2 Answers 0 Followers

Questions related from Aman Chahal

Are there any specific steps for immunolabelling of nuclear proteins versus cytosolic proteins?

I have attempted to detect a nuclear protein (RUNX2) and images by the supplier of the antibody shows the protein being present within the nucleus of the cell. I have used the primary in a 1:100...

11 August 2018 1,350 3 View

Has anyone immunolabelled for vinculin and talin/paxillin/FAK within the focal adhesion complex on the same sample?

I am interested in fluorescently imunolablelling more than one protein within the focal adhesion complex (vinculin, talin, FAK, or paxillin) and was curious if anyone has tried labeling two...

05 January 2017 5,097 2 View