How to amplify NCF1 with real time PCR without amplifying its pseudogenes NCF1B, NCF1C ?

I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !

05 July 2024 3,029 3 View