Amal Younis

2 Questions 2 Answers 0 Followers

Questions related from Amal Younis

How to accurately measure the autophagic flux using LC3 marker ?

Hello to all autophagy experts, or those who know a bit more deeply about autophagy. Currently, we are using LC3 to estimate the autophagic activity of HEK293T cells in different aggregative...

05 April 2022 1,099 2 View

Is there any recommended way to separate the aggregates of a mutant aggregative protein from the rest of the lysate ?

i'm working to get the aggregates generated from my mutant proteins separated from the rest of the cell's lysate.. i'll be glad to hear about recommended lysis buffers that can be serving this...

15 March 2018 4,305 4 View