What vector and B-catenin mutation should I use to generate a stable transfection of constitutively active nuclear B-catenin in RKO cells?

I am trying to generate RKO cells with constitutively active nuclear B-catenin. I have transfected with two different plasmids: pCl-neo beta catenin S33Y (addgene plasmid #16519) and...

10 October 2016 6,438 0 View