Aloysius Wilfred Raj Arokiaraj

3 Questions 5 Answers 0 Followers

Questions related from Aloysius Wilfred Raj Arokiaraj

Quantifying GABA and glutamate in fixed cell populations?

I am looking for a simple method for quantifying GABA and glutamate. I have a population of cells which are fixed and am interested in knowing the amount of these two neurotransmitters in the...

10 June 2016 934 4 View

How long can formalin-methanol fixed cells be in good condition for sorting and further RNA analysis?

I am planning to do flowcytometry on isolated cells from specific brain regions. Since I get a few thousand cells from 4-5 mice, I am thinking of fixing (formalin-methanol and store in -20degreeC)...

19 February 2016 2,459 2 View

Distance of RBS from the start codon in cell free expression system?

It is said the ribosome binding site (RBS) should be 6-7 base pairs in prokaryotic systems from the start codon ATG. But in my case I have a sequence which is "AGGAGA TACCCTT CAG GGA TCC ATG"...

03 September 2013 7,233 5 View