What is better to add first, ligand(agonist) or forskolin for cAMP HTRF for CCR2 (Gi Coupled)?

Hi Everyone, I am optimizing cAMP HTRF assay for the CCR2 receptor which couples to Gi. I wanted to know what is normally used and if you have experience in what works better for you. Adding the...

16 August 2023 7,623 1 View