Allyson Cochran

2 Questions 8 Answers 0 Followers

Questions related from Allyson Cochran

Alternate cell viability assay for MCF7 cells?

Hi All, I've been treating several cancer cell lines and a non-cancer cell line with WST-1, a variation of the MTT or MST viability assays, to monitor cell death with my immunotherapy drug. All...

11 March 2020 2,512 10 View

Dye front is separating into a "blob" - blue on top, purple on bottom?

I'm attempting to probe for low molecular weight proteins (11-25 kDa) from whole-cell lysates using a Tris based RIPA lysis buffer with inhibitors. I've been trying to separate these extracts...

04 December 2017 2,658 5 View