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I am doing immunofluorescence of mouse brain cryosections. I collect 25 um sections onto 1% gelatin coated coverslips, let them dry ~30 min, then peel off the OCT, rehdyrate the sections with 0.1...
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Hello, I am making cytoskeleton stabilizing buffer for the first time. The recipe I am using is 1 M NaCl, 100 mM PIPES, 30 mM MgCl2, 10 mM EGTA, 10 mM sucrose, in H2O. I made stock solutions of...
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