Alka Senwal

5 Questions 5 Answers 0 Followers

Questions related from Alka Senwal

Can somebody help calculating enzyme kinetics parameters with GC-MS method?

I am working on an enzyme and calculating the amount of product formed with GC-MS method. So I have calculated the amount of product frormed in uM from the area under curve of product and known...

06 September 2022 6,237 1 View

pEC86 plasmid map?

Hi everyone, Does anyone know which inducer is needed to overexpress pEC86 plasmid ? Has anyone used this plasmid to overexpress cytochromes and has details? Thanks

22 June 2021 8,558 3 View

The protein interaction from MST did not give the proper saturation curve?

I am studying protein-protein interactions and from MST, I observed the binding during binding check but I cannot get a full saturation anywhere with binding affinity tests. I tried changing the...

17 February 2021 7,209 4 View

Heme cofactor saturation in E. Coli?

I am working with mutiheme cytochrome c and I am not able to get full saturation of heme in my protein. Is there any growth supplements I can add to increase the saturation or any other...

24 September 2020 6,337 6 View

Recombinant cyt c expression in e.coli?

I overexpressed soluble cytochrome c in e.coli. After Ni-NTA. I am getting two protein bands on SDS-PAGE - the one which matches my theoretical protein molecular weight 40 kda is having a fainter...

04 January 2020 1,874 2 View