Alja Štern

5 Questions 4 Answers 0 Followers

Questions related from Alja Štern

Best/cheapest Ca2+ assay kit for Ca2+ measurement in complex media?

Has anyone a suggestion for a good Ca2+ detection colorimetric assay kit (96well) for measuring Ca2+ in very complex growth media containing lots of protein, minerals and oils... We used to...

11 November 2018 8,744 1 View

Soy concentrate hydrolyzate - turns green?

We have preformed numerous hydrolysis of soy concentrates (several different batches from different producers). We use standard conditions and mineral supplements. Last week one of the prepared...

07 July 2016 7,698 3 View

Enzymatic protein hydrolysis - how to calculate time of pH regulation to get desired pH drop at desired time?

I'm degrading soy protein with a alcalase and I have been measuring base consumption (while regulating the pH at the enzyme pH optimum), which according to literature is proportional to the amount...

03 March 2016 9,577 3 View

How do I choose the right protease concentration for protein hydrolysis from activity units/g?

I got samples of different enzymes in liquid form and the specification only gives the activity in U/g but I can’t figure out whether that means per g substrate proteine, per g enzyme or per g of...

11 November 2015 1,702 6 View

Witch wavelength is best for measuring protein in the Biuret test?

Witch wavelength is best for measuring protein in the Biuret test? I came across different variants ranging from 540-565nm, with the reagent consisting of the same chemicals. I tried measuring at...

11 November 2015 3,756 3 View