Alison Favaroni

3 Questions 4 Answers 0 Followers

Questions related from Alison Favaroni

T-test or one way ANOVA in RT-qPCR gene expression?

Hello, I checked the expression of several genes from different strains and I obtained the values of the relative normalized expression of each gene from each strain compared to the relative gene...

04 April 2019 6,438 4 View

How do i set up Biological replicates using CFX Maestro Software Bio-Rad?

Hello everybody, I am performing gene expression analysis using the Bio-Rad CFX maestro Software. I have different strains which I set up as "biological groups". In different plates I put my...

01 January 2019 9,282 1 View

Why do I get too high PCR efficiency?

Hello, I am performing RTqPCR, analyzing several bacterial genes for their expression. When I check the efficiency of the primers using gDNA serial dilutions, the efficiencies look ok, between...

01 January 1970 272 3 View