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Questions related from Alison Catalano
We have been developing an assay for sometime. However now we are about to go into validation we are questioning if it is good enough. 10% of the assays have random wells with high inter-well CV%....
05 May 2018 949 3 View
We wish to store antibody which will be at 2 - 2.5 mg/mL in a glycine buffer. We store at -80°C. If we store single use aliquots of 13 uL in screw cap o ringed vials (max volume 0.5mL) will they...
08 August 2017 9,693 5 View
If you were trying to do an ELISA with lower affinity antibodies what would you change? I know that ELISAs are meant to bias towards high affnity antibodies, but if you were aware that you were...
05 May 2017 2,249 5 View
The standard curve reagent in our validated assay is a polyclonal antibody. It is used to measure a drug substance that is also a polyclonal antibody. This is not measuring like we would like,...
10 October 2016 6,902 7 View
When developing a competitive ELISA how do you improve sensitivity? The format is to measure polyclonal antibody: a capture mAB on the plate, captures antigen, and then a labelled mAB is...
06 June 2016 381 11 View
When developing ELISA I have always followed the rule of coating just below saturation level. This avoids stacking issues. I would like to know if there are any consequences for using lower...
06 June 2014 2,786 10 View
